In the mitochondria where is the ph higher

Which of the following is not an effect of cyanide's inhibition of cytochrome C? The inhibition of cytochrome C means that the electron transport chain is no longer able to shuttle electrons from complex III to complex IV, which means it is no longer able to accept electrons from electron carriers. As a result, the citric acid cycle would slow down since there would be a build-up of NADH, which allosterically inhibits several enzymes in the citric acid cycle.

Since the electron transport chain no longer functions properly, there wouldn't be as many ions being pumped into the intermembrane space, which would increase the pH in the intermembrane space.

Also, with the decline in the concentration, oxidative phosphorylation would no longer be efficient, and the cell would have to increase rate of fermentation to increase energy output. If you've found an issue with this question, please let us know. With the help of the community we can continue to improve our educational resources. If Varsity Tutors takes action in response to an Infringement Notice, it will make a good faith attempt to contact the party that made such content available by means of the most recent email address, if any, provided by such party to Varsity Tutors.

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Correct answer: Electron transport chain proteins. Report an Error. Example Question 32 : Biochemistry And Metabolism. Which of the following areas of the mitochondria has the lowest pH? Possible Answers: The mitochondrial matrix. Correct answer: The intermembrane space. Explanation : ATP synthase, which is located on the inner mitochondrial membrane, requires a proton gradient in order to create ATP. Example Question 33 : Biochemistry And Metabolism. Possible Answers: Pyruvate decarboxylation Oxidative phosphorylation Citric acid cycle.

Correct answer: Pyruvate decarboxylation Citric acid cycle Oxidative phosphorylation. Explanation : After glycolysis is complete, we have generated pyruvate from glucose. Possible Answers:. A deficiency in any of these will limit the rate of oxidative phosphorylation. Correct answer: A deficiency in any of these will limit the rate of oxidative phosphorylation. Explanation : Oxidative phosphorylation is dependent on the functionality of the electron transport chain.

Example Question 35 : Biochemistry And Metabolism. Which of these processes in aerobic respiration would not be possible in the absence of oxygen? Possible Answers: Substrate-level phosphorylation. Correct answer: The electron transport chain. Explanation : Oxygen is necessary to be the last electron acceptor in the electron transport chain.

Example Question : Mcat Biological Sciences. Possible Answers: I only. Explanation : The two main classifications for transport are active transport and passive transport. Substrate-level phosphorylation would be inhibited. Pyruvate would be unable to enter the Krebs cycle. Example Question 38 : Biochemistry And Metabolism. When a certain bacterium undergoes aerobic respiration, which area would have the lowest pH?

O2, lactic acid, pyruvate , FADH2. Lactic acid, ethanol, CO2. In the absence of oxygen, the primary purpose of fermentation is to:.

The inside part analogues to the cytosol of a bacterium of a mitochondrion is called :. Porins may be found :. The terminal electron acceptor during mitochondrial respiration:.

During TCA cycle, the conversion of succinate to fumarate. Glyoxylate shunt. In the presence of an uncoupler , one may expect:. Which of the following statements about mitochondria is false? They contain an inner and an outer membrane. The region enclosed by the inner membrane is termed the matrix. They contain DNA and ribosomes.

They are an important site for energy production in cells. They contain stacked internal thylakoid membranes. If you isolate mitochondria and place them in buffer with a low pH they begin to manufacture ATP. Low pH increases the acid concentration in the mitochondrial matrix, a condition that normally causes ATP production. PCR primers were purchased from Sigma Aldrich.

Two days after the transfection with sEcGFP fusion constructs, the pH of the transfected cells was recorded in situ by confocal fluorescence emission ratio imaging inverted Olympus Fluoview FV All measurements were performed at room temperature in fresh growth medium. Ratio images were obtained simultaneously using the NIR laser ex. Nigericin was purchased from Enzo Life. Other commonly used chemicals were purchased from Roth. Thus, measurements were performed using equilibrated dye.

The fluorescence of mitochondrial TMRE in the mitochondrial regions of interest was then determined using the same settings gain and fluorescence excitation power in all samples.

Because the cells were transiently transfected, an internal control was always available. Then, a threshold level for the converted bit images was determined interactively for each cell. The background was set to NaN. The Boltzmann fit was chosen here for fitting the sigmoidal curves of the calibration data, in accordance with Grynkiewicz 38 , who described the relation between analyte concentration and fluorescence and fluorescence ratios. The fitting was performed using Origin 6.

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